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A cryptophane-based "turn-on" 129Xe NMR biosensor for monitoring calmodulin.

ABSTRACT: We present the first cryptophane-based "turn-on" 129Xe NMR biosensor, employing a peptide-functionalized cryptophane to monitor the activation of calmodulin (CaM) protein in solution. In the absence of CaM binding, interaction between the peptide and cryptophane completely suppresses the hyperpolarized 129Xe-cryptophane NMR signal. Biosensor binding to Ca2+-activated CaM produces the expected 129Xe-cryptophane NMR signal.

SUBMITTER: Riggle BA

PROVIDER: S-EPMC5681859 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature

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A cryptophane-based "turn-on" 129Xe NMR biosensor for monitoring calmodulin.

Riggle Brittany A BA Greenberg Mara L ML Wang Yanfei Y Wissner Rebecca F RF Zemerov Serge D SD Petersson E James EJ Dmochowski Ivan J IJ

Organic & biomolecular chemistry 20171001 42

We present the first cryptophane-based "turn-on" 129Xe NMR biosensor, employing a peptide-functionalized cryptophane to monitor the activation of calmodulin (CaM) protein in solution. In the absence of CaM binding, interaction between the peptide and cryptophane completely suppresses the hyperpolarized 129Xe-cryptophane NMR signal. Biosensor binding to Ca2+-activated CaM produces the expected 129Xe-cryptophane NMR signal. ...[more]

PMID: 29058007

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