Ontology highlight
ABSTRACT:
SUBMITTER: Galick HA
PROVIDER: S-EPMC5689654 | biostudies-literature | 2017 Oct
REPOSITORIES: biostudies-literature
Galick Heather A HA Marsden Carolyn G CG Kathe Scott S Dragon Julie A JA Volk Lindsay L Nemec Antonia A AA Wallace Susan S SS Prakash Aishwarya A Doublié Sylvie S Sweasy Joann B JB
Oncotarget 20170908 49
Base excision repair (BER) is a key genome maintenance pathway. The NEIL1 DNA glycosylase recognizes oxidized bases, and likely removes damage in advance of the replication fork. The rs5745906 SNP of the <i>NEIL1</i> gene is a rare human germline variant that encodes the NEIL1 G83D protein, which is devoid of DNA glycosylase activity. Here we show that expression of G83D NEIL1 in MCF10A immortalized but non-transformed mammary epithelial cells leads to replication fork stress. Upon treatment wit ...[more]