Ontology highlight
ABSTRACT:
SUBMITTER: Anthony JM
PROVIDER: S-EPMC5703184 | biostudies-literature | 2017 Nov
REPOSITORIES: biostudies-literature
Applications in plant sciences 20171121 11
<h4>Premise of the study</h4>Microsatellite markers were developed for population genetic analysis in the rare shrub <i>Styphelia longissima</i> (Ericaceae).<h4>Methods and results</h4>We generated ca. 2.5 million sequence reads using a Personal Genome Machine semiconductor sequencer. Using the QDD pipeline, we designed primers for >12,000 sequences with PCR product lengths of 80-480 bp. From these, 30 primer pairs were selected and screened using PCR; of these, 16 loci were found to be polymorp ...[more]