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Development and characterization of microsatellite markers in Gaultheria pumila Lf. (Ericaceae).

ABSTRACT: BACKGROUND:Polymorphic microsatellite markers were developed for Gaultheria pumila (Ericaceae) to evaluate genetic diversity and population structure within its native range in Chile. This is a very important Ericaceae endemic to Chile with a large commercial potential. Its resistance to different abiotic conditions makes it a valuable target for genetic improvement. RESULTS:Ten polymorphic simple sequence repeat (SSR) loci were isolated from Gaultheria pumila using new-generation 454 FLX Titanium pyrosequencing technology. The mean number of alleles per locus ranged from 2 to 4. Observed and expected heterozygosity ranged from 0.00 to 1.0 and 0.00 to 0.64, respectively. CONCLUSIONS:From 10 SSR markers developed for G. pumila, 9 markers are promising candidates for analyzing genetic variation within or between natural populations of G. pumila and other species from the same genus.

SUBMITTER: Garcia-Gonzales R 

PROVIDER: S-EPMC6211410 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature

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Development and characterization of microsatellite markers in Gaultheria pumila Lf. (Ericaceae).

Garcia-Gonzales Rolando R   Pico-Mendoza José J   Quiroz Karla K   Carrasco Basilio B   Cáceres Pablo P   Chong-Perez Borys B   Pino Hugo H   Seiltgens Marjorie M   Greck Eglis E   Caligari Peter D S PDS  

Biological research 20181101 1

<h4>Background</h4>Polymorphic microsatellite markers were developed for Gaultheria pumila (Ericaceae) to evaluate genetic diversity and population structure within its native range in Chile. This is a very important Ericaceae endemic to Chile with a large commercial potential. Its resistance to different abiotic conditions makes it a valuable target for genetic improvement.<h4>Results</h4>Ten polymorphic simple sequence repeat (SSR) loci were isolated from Gaultheria pumila using new-generation  ...[more]

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