Ontology highlight
ABSTRACT:
SUBMITTER: Hustedt N
PROVIDER: S-EPMC5706562 | biostudies-literature | 2015 Jan
REPOSITORIES: biostudies-literature
Hustedt Nicole N Seeber Andrew A Sack Ragna R Tsai-Pflugfelder Monika M Bhullar Bhupinder B Vlaming Hanneke H van Leeuwen Fred F Guénolé Aude A van Attikum Haico H Srivas Rohith R Ideker Trey T Shimada Kenji K Gasser Susan M SM
Molecular cell 20141218 2
Mec1-Ddc2 (ATR-ATRIP) controls the DNA damage checkpoint and shows differential cell-cycle regulation in yeast. To find regulators of Mec1-Ddc2, we exploited a mec1 mutant that retains catalytic activity in G2 and recruitment to stalled replication forks, but which is compromised for the intra-S phase checkpoint. Two screens, one for spontaneous survivors and an E-MAP screen for synthetic growth effects, identified loss of PP4 phosphatase, pph3Δ and psy2Δ, as the strongest suppressors of mec1-10 ...[more]