Model-based genome-wide determination of RNA chain elongation rates in Escherichia coli.

ABSTRACT: Dynamics in the process of transcription are often simplified, yet they play an important role in transcript folding, translation into functional protein and DNA supercoiling. While the modulation of the speed of transcription of individual genes and its role in regulation and proper protein folding has been analyzed in depth, the functional relevance of differences in transcription speeds as well as the factors influencing it have not yet been determined on a genome-wide scale. Here we determined transcription speeds for the majority of E. coli genes based on experimental data. We find large differences in transcription speed between individual genes and a strong influence of both cellular location as well as the relative importance of genes for cellular function on transcription speeds.

SUBMITTER: Großmann P 

PROVIDER: S-EPMC5722913 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature