Unknown

Dataset Information

0

Transcriptome-wide Analysis of Roles for tRNA Modifications in Translational Regulation.


ABSTRACT: Covalent nucleotide modifications in noncoding RNAs affect a plethora of biological processes, and new functions continue to be discovered even for well-known modifying enzymes. To systematically compare the functions of a large set of noncoding RNA modifications in gene regulation, we carried out ribosome profiling in budding yeast to characterize 57 nonessential genes involved in tRNA modification. Deletion mutants exhibited a range of translational phenotypes, with enzymes known to modify anticodons, or non-tRNA substrates such as rRNA, exhibiting the most dramatic translational perturbations. Our data build on prior reports documenting translational upregulation of the nutrient-responsive transcription factor Gcn4 in response to numerous tRNA perturbations, and identify many additional translationally regulated mRNAs throughout the yeast genome. Our data also uncover unexpected roles for tRNA-modifying enzymes in regulation of TY retroelements, and in rRNA 2'-O-methylation. This dataset should provide a rich resource for discovery of additional links between tRNA modifications and gene regulation.

SUBMITTER: Chou HJ 

PROVIDER: S-EPMC5728682 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature

altmetric image

Publications

Transcriptome-wide Analysis of Roles for tRNA Modifications in Translational Regulation.

Chou Hsin-Jung HJ   Donnard Elisa E   Gustafsson H Tobias HT   Garber Manuel M   Rando Oliver J OJ  

Molecular cell 20171130 5


Covalent nucleotide modifications in noncoding RNAs affect a plethora of biological processes, and new functions continue to be discovered even for well-known modifying enzymes. To systematically compare the functions of a large set of noncoding RNA modifications in gene regulation, we carried out ribosome profiling in budding yeast to characterize 57 nonessential genes involved in tRNA modification. Deletion mutants exhibited a range of translational phenotypes, with enzymes known to modify ant  ...[more]

Similar Datasets

2017-06-29 | GSE100626 | GEO
| S-EPMC10888749 | biostudies-literature
| S-EPMC9688753 | biostudies-literature
| S-EPMC7937688 | biostudies-literature
| S-EPMC6658992 | biostudies-literature
| S-EPMC9690228 | biostudies-literature
| S-EPMC6801620 | biostudies-literature
| S-EPMC3495739 | biostudies-literature
| S-EPMC9775095 | biostudies-literature
| S-EPMC8505795 | biostudies-literature