Ontology highlight
ABSTRACT:
SUBMITTER: Wang P
PROVIDER: S-EPMC5737490 | biostudies-literature | 2017 Nov
REPOSITORIES: biostudies-literature
Wang Pingping P Byrum Stephanie S Fowler Faith C FC Pal Sangita S Tackett Alan J AJ Tyler Jessica K JK
Nucleic acids research 20171101 19
Here, we use ChAP-MS (chromatin affinity purification with mass spectrometry), for the affinity purification of a sequence-specific single-copy endogenous chromosomal locus containing a DNA double-strand break (DSB). We found multiple new histone post-translational modifications enriched on chromatin bearing a DSB from budding yeast. One of these, methylation of histone H3 on lysine 125, has not previously been reported. Among over 100 novel proteins enriched at a DSB were the phosphatase Sit4, ...[more]