Ontology highlight
ABSTRACT:
SUBMITTER: Flett FJ
PROVIDER: S-EPMC5750209 | biostudies-literature | 2018 Jan
REPOSITORIES: biostudies-literature
Flett Fiona J FJ Ruksenaite Emilija E Armstrong Lee A LA Bharati Shipra S Carloni Roberta R Morris Elizabeth R ER Mackay C Logan CL Interthal Heidrun H Richardson Julia M JM
Nature communications 20180102 1
Tyrosyl-DNA phosphodiesterase (Tdp1) is a DNA 3'-end processing enzyme that repairs topoisomerase 1B-induced DNA damage. We use a new tool combining site-specific DNA-protein cross-linking with mass spectrometry to identify Tdp1 interactions with DNA. A conserved phenylalanine (F259) of Tdp1, required for efficient DNA processing in biochemical assays, cross-links to defined positions in DNA substrates. Crystal structures of Tdp1-DNA complexes capture the DNA repair machinery after 3'-end cleava ...[more]