Ontology highlight
ABSTRACT:
SUBMITTER: Adhikari S
PROVIDER: S-EPMC3125436 | biostudies-literature | 2011 Sep
REPOSITORIES: biostudies-literature
Adhikari Sanjay S Karmahapatra Soumendra K SK Elias Hadi H Dhopeshwarkar Priyanka P Williams R Scott RS Byers Stephen S Uren Aykut A Roy Rabindra R
Analytical biochemistry 20110512 1
Tyrosyl DNA phosphodiesterase 2 (TDP2), a newly discovered enzyme that cleaves 5'-phosphotyrosyl bonds, is a potential target for chemotherapy. TDP2 possesses both 3'- and 5'-tyrosyl-DNA phosphodiesterase activity, which is generally measured in a gel-based assay using 3'- and 5'-phosphotyrosyl linkage at the 3' and 5' ends of an oligonucleotide. To understand the enzymatic mechanism of this novel enzyme, the gel-based assay is useful, but this technique is cumbersome for TDP2 inhibitor screenin ...[more]