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Term-seq reveals abundant ribo-regulation of antibiotics resistance in bacteria.


ABSTRACT: Riboswitches and attenuators are cis-regulatory RNA elements, most of which control bacterial gene expression via metabolite-mediated, premature transcription termination. We developed an unbiased experimental approach for genome-wide discovery of such ribo-regulators in bacteria. We also devised an experimental platform that quantitatively measures the in vivo activity of all such regulators in parallel and enables rapid screening for ribo-regulators that respond to metabolites of choice. Using this approach, we detected numerous antibiotic-responsive ribo-regulators that control antibiotic resistance genes in pathogens and in the human microbiome. Studying one such regulator in Listeria monocytogenes revealed an attenuation mechanism mediated by antibiotic-stalled ribosomes. Our results expose broad roles for conditional termination in regulating antibiotic resistance and provide a tool for discovering riboswitches and attenuators that respond to previously unknown ligands.

SUBMITTER: Dar D 

PROVIDER: S-EPMC5756622 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature

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Term-seq reveals abundant ribo-regulation of antibiotics resistance in bacteria.

Dar Daniel D   Shamir Maya M   Mellin J R JR   Koutero Mikael M   Stern-Ginossar Noam N   Cossart Pascale P   Sorek Rotem R  

Science (New York, N.Y.) 20160401 6282


Riboswitches and attenuators are cis-regulatory RNA elements, most of which control bacterial gene expression via metabolite-mediated, premature transcription termination. We developed an unbiased experimental approach for genome-wide discovery of such ribo-regulators in bacteria. We also devised an experimental platform that quantitatively measures the in vivo activity of all such regulators in parallel and enables rapid screening for ribo-regulators that respond to metabolites of choice. Using  ...[more]

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