Ontology highlight
ABSTRACT:
SUBMITTER: Mi W
PROVIDER: S-EPMC5759761 | biostudies-literature | 2017 Sep
REPOSITORIES: biostudies-literature
Mi Wei W Li Yanyan Y Yoon Sung Hwan SH Ernst Robert K RK Walz Thomas T Liao Maofu M
Nature 20170906 7671
Lipopolysaccharide (LPS) in the outer membrane of Gram-negative bacteria is critical for the assembly of their cell envelopes. LPS synthesized in the cytoplasmic leaflet of the inner membrane is flipped to the periplasmic leaflet by MsbA, an ATP-binding cassette transporter. Despite substantial efforts, the structural mechanisms underlying MsbA-driven LPS flipping remain elusive. Here we use single-particle cryo-electron microscopy to elucidate the structures of lipid-nanodisc-embedded MsbA in t ...[more]