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Two-color vibrational imaging of glucose metabolism using stimulated Raman scattering.


ABSTRACT: A two-color vibrational imaging technique for simultaneously mapping glucose uptake and incorporation activity inside single living cells is reported. Heterogeneous patterns of glucose metabolism are directly visualized from the ratiometric two-color images for various cell types, cells undergoing epithelia-to-mesenchymal transitions and live mouse brain tissues. The two-color imaging of glucose metabolism here demonstrates the development of multi-functional vibrational probes for multicolor imaging of cellular metabolism.

SUBMITTER: Long R 

PROVIDER: S-EPMC5764084 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature

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Two-color vibrational imaging of glucose metabolism using stimulated Raman scattering.

Long Rong R   Zhang Luyuan L   Shi Lingyan L   Shen Yihui Y   Hu Fanghao F   Zeng Chen C   Min Wei W  

Chemical communications (Cambridge, England) 20171208 2


A two-color vibrational imaging technique for simultaneously mapping glucose uptake and incorporation activity inside single living cells is reported. Heterogeneous patterns of glucose metabolism are directly visualized from the ratiometric two-color images for various cell types, cells undergoing epithelia-to-mesenchymal transitions and live mouse brain tissues. The two-color imaging of glucose metabolism here demonstrates the development of multi-functional vibrational probes for multicolor im  ...[more]

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