Sensing Native Protein Solution Structures Using a Solid-state Nanopore: Unraveling the States of VEGF.

ABSTRACT: Monitoring individual proteins in solution while simultaneously obtaining tertiary and quaternary structural information is challenging. In this study, translocation of the vascular endothelial growth factor (VEGF) protein through a solid-state nanopore (ssNP) produces distinct ion-current blockade amplitude levels and durations likely corresponding to monomer, dimer, and higher oligomeric states. Upon changing from a non-reducing to a reducing condition, ion-current blockage events from the monomeric state dominate, consistent with the expected reduction of the two inter-chain VEGF disulfide bonds. Cleavage by plasmin and application of either a positive or a negative NP bias results in nanopore signals corresponding either to the VEGF receptor recognition domain or to the heparin binding

SUBMITTER: Varongchayakul N 

PROVIDER: S-EPMC5772516 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature