Analyzing the Catalytic Activities and Interactions of Eukaryotic Translesion Synthesis Polymerases.

ABSTRACT: Translesion synthesis is the process by which nonclassical DNA polymerases bypass DNA damage during DNA replication. Cells possess a variety of nonclassical polymerases, each one is specific for incorporating nucleotides opposite to one or more closely related DNA lesions, called its cognate lesions. In this chapter, we discuss a variety of approaches for probing the catalytic activities and the protein-protein interactions of nonclassical polymerases. With respect to their catalytic activities, we discuss polymerase assays, steady-state kinetics, and presteady-state kinetics. With respect to their interactions, we discuss qualitative binding assays such as enzyme-linked immunosorbent assays and coimmunoprecipitation; quantitative binding assays such as isothermal titration calorimetry, su

SUBMITTER: Powers KT 

PROVIDER: S-EPMC5772771 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature