Ontology highlight
ABSTRACT:
SUBMITTER: Liu H
PROVIDER: S-EPMC5777047 | biostudies-literature | 2018 Jan
REPOSITORIES: biostudies-literature
Liu Hui H Dong Peng P Ioannou Maria S MS Li Li L Shea Jamien J Pasolli H Amalia HA Grimm Jonathan B JB Rivlin Patricia K PK Lavis Luke D LD Koyama Minoru M Liu Zhe Z
Proceedings of the National Academy of Sciences of the United States of America 20171228 2
Our ability to unambiguously image and track individual molecules in live cells is limited by packing of multiple copies of labeled molecules within the resolution limit. Here we devise a universal genetic strategy to precisely control copy number of fluorescently labeled molecules in a cell. This system has a dynamic range of ∼10,000-fold, enabling sparse labeling of proteins expressed at different abundance levels. Combined with photostable labels, this system extends the duration of automated ...[more]