Dataset Information

Correlation between hepatic human males absent on the first (hMOF) and viral persistence in chronic hepatitis B patients.

ABSTRACT:

Background

Chronic hepatitis B (CHB) remains a global health dilemma with high morbidity and mortality. Human males absent on the first (hMOF) (a histone acetyltransferase) is responsible for DNA damage repair, tumorigenesis and cell cycle regulation. Persistence of HBV DNA contributes to cirrhosis and hepatocellular carcinoma (HCC) in CHB patients. Histone acetyltransferase enhances HBV replication, however the precise underlying mechanism of hMOF in HBV replication in CHB patients remains to be explored. This study aims to investigate the correlation between hepatic hMOF and HBV DNA replication in CHB patients, and may provide new insights towards the treatment of CHB patients.

Methods

hMOF in liver biopsy (CHB, n = 33 HBeAg⁺; n = 20 HBeAg^-, and three healthy controls) was determined, using immunohistochemistry, qPCR and Western blot. The correlation between hMOF and HBsAg, as well as, HBeAg were determined.

Results

A positive correlation between hMOF and HBV DNA in overall CHB patients was observed. A distinct positive correlation between hMOF and HBsAg and/or HBeAg in HBeAg⁺ CHB patients was also detected, however not observed between hMOF and HBsAg in HBeAg^- CHB patients. No correlation was observed between hMOF and hepatic inflammation severity and fibrotic stage in CHB patients.

Conclusions

Hepatic hMOF might contribute to host HBV clearance in CHB patients and possible pathogenesis.

SUBMITTER: Chen L

PROVIDER: S-EPMC5819663 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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Publications

Correlation between hepatic human males absent on the first (hMOF) and viral persistence in chronic hepatitis B patients.

Chen Liwen L Zhu Chuanwu C Li Fengdi F Wang Yun Y Bao Rebecca R Cao Zhujun Z Xiang Xiaogang X Yan Lei L Lin Lanyi L Zhao Gangde G Xie Qing Q Bao Shisan S Wang Hui H

Cell & bioscience 20180220

<h4>Background</h4>Chronic hepatitis B (CHB) remains a global health dilemma with high morbidity and mortality. Human males absent on the first (hMOF) (a histone acetyltransferase) is responsible for DNA damage repair, tumorigenesis and cell cycle regulation. Persistence of HBV DNA contributes to cirrhosis and hepatocellular carcinoma (HCC) in CHB patients. Histone acetyltransferase enhances HBV replication, however the precise underlying mechanism of hMOF in HBV replication in CHB patients rema ...[more]

PMID: 29484170

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Project description:AIM:To investigate the evaluation of hepatitis C virus (HCV) quasispecies in the envelope region and its relationship with the outcome of acute hepatitis C. METHODS:HCV quasispecies were characterized in specimens collected every 2-6 mo from a cohort of acutely HCV-infected subjects. We evaluated two individuals who spontaneously cleared viremia and three individuals with persistent viremia by cloning 33 1-kb amplicons that spanned E1 and the 5' half of E2, including hypervariable region 1 (HVR1). To assess the quasispecies complexity and to detect variants for sequencing, 33 cloned cDNAs representing each specimen were assessed by a combined method of analysis of a single-stranded conformational polymorphism and heteroduplex analysis. The rates of both synonymous and nonsynonymous substitutions for the E1, HVR1 and E2 regions outside HVR1 were analyzed. RESULTS:Serum samples collected from chronic phase of infection had higher quasispecies complexity than those collected from acute phase of infection in all individuals examined. The genetic diversity (genetic distance) within HVR1 was consistently higher than that in the complete E1 (0.0322+/-0.0068 vs -0.0020+/-0.0014, P<0.05) and E2 regions outside HVR1 (0.0322+/-0.0068 vs 0.0017+/-0.0011, P<0.05) in individuals with persistent viremia, but did not change markedly over time in those with clearance of viremia. For individuals with persistent viremia, the rate of nonsynonymous substitutions within the HVR1 region (2.76X10(-3)+/-1.51X10(-3)) predominated and gradually increased, as compared with that in the E1 and E2 regions outside HVR1 (0.23X10(-3)+/-0.15X10(-3), 0.50X10(-3)+/-0.10X10(-3)). By contrast, the rates of both nonsynonymous and synonymous substitutions for the E1 and E2 regions including HVR1 were consistently lower in individuals with clearance of viremia. CONCLUSION:HCV persistence is associated with a complexity quasispecies and positive selection of HVR1 by the host immune system.

Dataset Information

Correlation between hepatic human males absent on the first (hMOF) and viral persistence in chronic hepatitis B patients.

Background

Methods

Results

Conclusions

Publications

Correlation between hepatic human males absent on the first (hMOF) and viral persistence in chronic hepatitis B patients.

Similar Datasets

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