Ontology highlight
ABSTRACT:
SUBMITTER: Lee J
PROVIDER: S-EPMC5824020 | biostudies-literature | 2018 Feb
REPOSITORIES: biostudies-literature
Lee Junwon J Choi Sang-Hwi SH Lee Dongjin R DR Kim Dae-Sung DS Kim Dong-Wook DW
Molecules and cells 20180131 2
The objective of this study was to induce the production of isthmic organizer (IsO)-like cells capable of secreting fibroblast growth factor (FGF) 8 and WNT1 from human embryonic stem cells (ESCs). The precise modulation of canonical Wnt signaling was achieved in the presence of the small molecule CHIR99021 (0.6 μM) during the neural induction of human ESCs, resulting in the differentiation of these cells into IsO-like cells having a midbrain-hindbrain border (MHB) fate in a manner that recapitu ...[more]