Unknown

Dataset Information

0

Structure of the yeast spliceosomal postcatalytic P complex.

ABSTRACT: The spliceosome undergoes dramatic changes in a splicing cycle. Structures of B, Bact, C, C*, and intron lariat spliceosome complexes revealed mechanisms of 5'-splice site (ss) recognition, branching, and intron release, but lacked information on 3'-ss recognition, exon ligation, and exon release. Here we report a cryo-electron microscopy structure of the postcatalytic P complex at 3.3-angstrom resolution, revealing that the 3' ss is mainly recognized through non-Watson-Crick base pairing with the 5' ss and branch point. Furthermore, one or more unidentified proteins become stably associated with the P complex, securing the 3' exon and potentially regulating activity of the helicase Prp22. Prp22 binds nucleotides 15 to 21 in the 3' exon, enabling it to pull the intron-exon or ligated exons in a 3' to 5' direction to achieve 3'-ss proofreading or exon release, respectively.

SUBMITTER: Liu S 

PROVIDER: S-EPMC5828012 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

Structure of the yeast spliceosomal postcatalytic P complex.

Liu Shiheng S   Li Xueni X   Zhang Lingdi L   Jiang Jiansen J   Hill Ryan C RC   Cui Yanxiang Y   Hansen Kirk C KC   Zhou Z Hong ZH   Zhao Rui R  

Science (New York, N.Y.) 20171116 6368

The spliceosome undergoes dramatic changes in a splicing cycle. Structures of B, B<sup>act</sup>, C, C*, and intron lariat spliceosome complexes revealed mechanisms of 5'-splice site (ss) recognition, branching, and intron release, but lacked information on 3'-ss recognition, exon ligation, and exon release. Here we report a cryo-electron microscopy structure of the postcatalytic P complex at 3.3-angstrom resolution, revealing that the 3' ss is mainly recognized through non-Watson-Crick base pai  ...[more]

Similar Datasets

Unknown Protein localisation by electron microscopy reveals the architecture of the yeast spliceosomal B complex.
| S-EPMC4687791 | biostudies-literature
Unknown Structure of spliceosomal ribonucleoproteins.
| S-EPMC2950031 | biostudies-other
Unknown The spliceosomal PRP19 complex of trypanosomes.
| S-EPMC4374492 | biostudies-literature
Unknown Molecular evolution of eukaryotic genomes: hemiascomycetous yeast spliceosomal introns.
| S-EPMC150231 | biostudies-literature
Unknown Use of the spliceosomal protein U1A to facilitate crystallization and structure determination of complex RNAs.
| S-EPMC2974902 | biostudies-literature
Unknown CryoEM structure of yeast cytoplasmic exosome complex.
| S-EPMC5129878 | biostudies-literature
Unknown The structure of the yeast Ctf3 complex.
| S-EPMC6602579 | biostudies-literature
Unknown Structure of the yeast U2/U6 snRNA complex.
| S-EPMC3312555 | biostudies-literature
Unknown Crystal structure of a core spliceosomal protein interface.
| S-EPMC1360545 | biostudies-literature
Unknown Integrative structure and function of the yeast exocyst complex.
| S-EPMC7255525 | biostudies-literature