Unknown

Dataset Information

0

PV1, a novel Plasmodium falciparum merozoite dense granule protein, interacts with exported protein in infected erythrocytes.


ABSTRACT: Upon invasion, Plasmodium falciparum exports hundreds of proteins across its surrounding parasitophorous vacuole membrane (PVM) to remodel the infected erythrocyte. Although this phenomenon is crucial for the parasite growth and virulence, elucidation of precise steps in the export pathway is still required. A translocon protein complex, PTEX, is the only known pathway that mediates passage of exported proteins across the PVM. P. falciparum Parasitophorous Vacuolar protein 1 (PfPV1), a previously reported parasitophorous vacuole (PV) protein, is considered essential for parasite growth. In this study, we characterized PfPV1 as a novel merozoite dense granule protein. Structured illumination microscopy (SIM) analyses demonstrated that PfPV1 partially co-localized with EXP2, suggesting the protein could be a PTEX accessory molecule. Furthermore, PfPV1 and exported protein PTP5 co-immunoprecipitated with anti-PfPV1 antibody. Surface plasmon resonance (SPR) confirmed the proteins' direct interaction. Additionally, we identified a PfPV1 High-affinity Region (PHR) at the C-terminal side of PTP5 where PfPV1 dominantly bound. SIM analysis demonstrated an export arrest of PTP5?PHR, a PTP5 mutant lacking PHR, suggesting PHR is essential for PTP5 export to the infected erythrocyte cytosol. The overall results suggest that PfPV1, a novel dense granule protein, plays an important role in protein export at PV.

SUBMITTER: Morita M 

PROVIDER: S-EPMC5829233 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature

altmetric image

Publications

PV1, a novel Plasmodium falciparum merozoite dense granule protein, interacts with exported protein in infected erythrocytes.

Morita Masayuki M   Nagaoka Hikaru H   Ntege Edward H EH   Kanoi Bernard N BN   Ito Daisuke D   Nakata Takahiro T   Lee Ji-Won JW   Tokunaga Kazuaki K   Iimura Tadahiro T   Torii Motomi M   Tsuboi Takafumi T   Takashima Eizo E  

Scientific reports 20180227 1


Upon invasion, Plasmodium falciparum exports hundreds of proteins across its surrounding parasitophorous vacuole membrane (PVM) to remodel the infected erythrocyte. Although this phenomenon is crucial for the parasite growth and virulence, elucidation of precise steps in the export pathway is still required. A translocon protein complex, PTEX, is the only known pathway that mediates passage of exported proteins across the PVM. P. falciparum Parasitophorous Vacuolar protein 1 (PfPV1), a previousl  ...[more]

Similar Datasets

| S-EPMC4817195 | biostudies-literature
| S-EPMC2253348 | biostudies-literature
| S-EPMC3436795 | biostudies-literature
| S-EPMC7075938 | biostudies-literature
| S-EPMC2593181 | biostudies-literature
| S-EPMC2568870 | biostudies-literature
| S-EPMC6977125 | biostudies-literature
| S-EPMC4270784 | biostudies-literature
| S-EPMC154316 | biostudies-literature
| S-EPMC4111544 | biostudies-literature