Ontology highlight
ABSTRACT:
SUBMITTER: Velmurugu Y
PROVIDER: S-EPMC5829579 | biostudies-literature | 2018 Feb
REPOSITORIES: biostudies-literature
Velmurugu Yogambigai Y Vivas Paula P Connolly Mitchell M Kuznetsov Serguei V SV Rice Phoebe A PA Ansari Anjum A
Nucleic acids research 20180201 4
The dynamics and mechanism of how site-specific DNA-bending proteins initially interrogate potential binding sites prior to recognition have remained elusive for most systems. Here we present these dynamics for Integration Host factor (IHF), a nucleoid-associated architectural protein, using a μs-resolved T-jump approach. Our studies show two distinct DNA-bending steps during site recognition by IHF. While the faster (∼100 μs) step is unaffected by changes in DNA or protein sequence that alter a ...[more]