Ontology highlight
ABSTRACT:
SUBMITTER: Platt RJ
PROVIDER: S-EPMC4265475 | biostudies-literature | 2014 Oct
REPOSITORIES: biostudies-literature
Cell 20140925 2
CRISPR-Cas9 is a versatile genome editing technology for studying the functions of genetic elements. To broadly enable the application of Cas9 in vivo, we established a Cre-dependent Cas9 knockin mouse. We demonstrated in vivo as well as ex vivo genome editing using adeno-associated virus (AAV)-, lentivirus-, or particle-mediated delivery of guide RNA in neurons, immune cells, and endothelial cells. Using these mice, we simultaneously modeled the dynamics of KRAS, p53, and LKB1, the top three si ...[more]