Ontology highlight
ABSTRACT:
SUBMITTER: Zambaldo C
PROVIDER: S-EPMC5847277 | biostudies-literature | 2017 Aug
REPOSITORIES: biostudies-literature
Zambaldo Claudio C Luo Xiaozhou X Mehta Angad P AP Schultz Peter G PG
Journal of the American Chemical Society 20170815 34
Nisin is a complex lanthipeptide that has broad spectrum antibacterial activity. In efforts to broaden the structural diversity of this ribosomally synthesized lantibiotic, we now report the recombinant expression of Nisin variants that incorporate noncanonical amino acids (ncAAs) at discrete positions. This is achieved by expressing the nisA structural gene, cyclase (nisC) and dehydratase (nisB), together with an orthogonal nonsense suppressor tRNA/aminoacyl-tRNA synthetase pair in Escherichia ...[more]