Contribution of major FLM isoforms to temperature-dependent flowering in Arabidopsis thaliana.

ABSTRACT: FLOWERING LOCUS M (FLM), a component of the thermosensory flowering time pathway in Arabidopsis thaliana, is regulated by temperature-dependent alternative splicing (AS). The main splicing variant, FLM-?, is a well-documented floral repressor that is down-regulated in response to increasing ambient growth temperature. Two hypotheses have been formulated to explain how flowering time is modulated by AS of FLM. In the first model a second splice variant, FLM-?, acts as a dominant negative isoform that competes with FLM-? at elevated ambient temperatures, thereby indirectly promoting flowering. Alternatively, it has been suggested that the induction of flowering at elevated temperatures is caused only by reduced FLM-? expression. To better understand the role of the two FLM splice forms, we employed CRISPR/Cas9 technology to specifically delete the exons that characterize each splice variant. Lines that produced repressive FLM-? but were incapable of producing FLM-? were late flowering. In contrast, FLM-? knockout lines that still produced FLM-? flowered early, but not earlier than the flm-3 loss of function mutant, as would be expected if FLM-? had a dominant-negative effect on flowering. Our data support the role of FLM-? as a flower repressor and provide evidence that a contribution of FLM-? to the regulation of flowering time in wild-type A. thaliana seems unlikely.

SUBMITTER: Capovilla G 

PROVIDER: S-EPMC5853260 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature