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Inorganic phosphate, arsenate, and vanadate enhance exonuclease transcript cleavage by RNA polymerase by 2000-fold.


ABSTRACT: Inorganic Pi is involved in all major biochemical pathways. Here we describe a previously unreported activity of Pi We show that Pi and its structural mimics, vanadate and arsenate, enhance nascent transcript cleavage by RNA polymerase (RNAP). They engage an Mg2+ ion in catalysis and activate an attacking water molecule. Pi, vanadate, and arsenate stimulate the intrinsic exonuclease activity of the enzyme nearly 2,000-fold at saturating concentrations of the reactant anions and Mg2+ This enhancement is comparable to that of specialized transcript cleavage protein factors Gre and TFIIS (3,000- to 4,000-fold). Unlike these protein factors, Pi and its analogs do not stimulate endonuclease transcript cleavage. Conversely, the protein factors only marginally enhance exonucleolytic cleavage. Pi thus complements cellular protein factors in assisting hydrolytic RNA cleavage by extending the repertoire of RNAP transcript degradation modes.

SUBMITTER: Gottesman ME 

PROVIDER: S-EPMC5856549 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature

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Inorganic phosphate, arsenate, and vanadate enhance exonuclease transcript cleavage by RNA polymerase by 2000-fold.

Gottesman Max E ME   Mustaev Arkady A  

Proceedings of the National Academy of Sciences of the United States of America 20180226 11


Inorganic P<sub>i</sub> is involved in all major biochemical pathways. Here we describe a previously unreported activity of P<sub>i</sub> We show that P<sub>i</sub> and its structural mimics, vanadate and arsenate, enhance nascent transcript cleavage by RNA polymerase (RNAP). They engage an Mg<sup>2+</sup> ion in catalysis and activate an attacking water molecule. P<sub>i</sub>, vanadate, and arsenate stimulate the intrinsic exonuclease activity of the enzyme nearly 2,000-fold at saturating conc  ...[more]

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