Ontology highlight
ABSTRACT:
SUBMITTER: Gottesman ME
PROVIDER: S-EPMC5856549 | biostudies-literature | 2018 Mar
REPOSITORIES: biostudies-literature
Gottesman Max E ME Mustaev Arkady A
Proceedings of the National Academy of Sciences of the United States of America 20180226 11
Inorganic P<sub>i</sub> is involved in all major biochemical pathways. Here we describe a previously unreported activity of P<sub>i</sub> We show that P<sub>i</sub> and its structural mimics, vanadate and arsenate, enhance nascent transcript cleavage by RNA polymerase (RNAP). They engage an Mg<sup>2+</sup> ion in catalysis and activate an attacking water molecule. P<sub>i</sub>, vanadate, and arsenate stimulate the intrinsic exonuclease activity of the enzyme nearly 2,000-fold at saturating conc ...[more]