Ontology highlight
ABSTRACT:
SUBMITTER: Ka Man Tse C
PROVIDER: S-EPMC6548511 | biostudies-literature | 2019 Mar
REPOSITORIES: biostudies-literature
Ka Man Tse Carmen C Xu Jun J Xu Liang L Sheong Fu Kit FK Wang Shenglong S Chow Hoi Yee HY Gao Xin X Li Xuechen X Cheung Peter Pak-Hang PP Wang Dong D Zhang Yingkai Y Huang Xuhui X
Nature energy 20190211 3
RNA polymerase II (Pol II) utilises the same active site for polymerization and intrinsic cleavage. Pol II proofreads the nascent transcript by its intrinsic nuclease activity to maintain high transcriptional fidelity critical for cell growth and viability. The detailed catalytic mechanism of intrinsic cleavage remains unknown. Here, we combined <i>ab initio</i> quantum mechanics/molecular mechanics studies and biochemical cleavage assays to show that Pol II utilises downstream phosphate oxygen ...[more]