Ontology highlight
ABSTRACT:
SUBMITTER: Repetto MV
PROVIDER: S-EPMC5858200 | biostudies-literature | 2018 Mar
REPOSITORIES: biostudies-literature
Repetto María Victoria MV Winters Matthew J MJ Bush Alan A Reiter Wolfgang W Hollenstein David Maria DM Ammerer Gustav G Pryciak Peter M PM Colman-Lerner Alejandro A
Molecular cell 20180301 6
We report an unanticipated system of joint regulation by cyclin-dependent kinase (CDK) and mitogen-activated protein kinase (MAPK), involving collaborative multi-site phosphorylation of a single substrate. In budding yeast, the protein Ste5 controls signaling through a G1 arrest pathway. Upon cell-cycle entry, CDK inhibits Ste5 via multiple phosphorylation sites, disrupting its membrane association. Using quantitative time-lapse microscopy, we examined Ste5 membrane recruitment dynamics at diffe ...[more]