Unknown

Dataset Information

0

Frequency of occurrence of HIV-1 dual infection in a Belgian MSM population.


ABSTRACT:

Introduction

HIV-1 dual infection is a condition that results from infection with at least two HIV-1 variants from different sources. The scarceness of information on this condition is partly due to the fact that its detection is technically challenging. Using next-generation sequencing we defined the extent of HIV-1 dual infection in a cohort of men who have sex with men (MSM).

Material & methods

Eighty-six MSM, diagnosed with HIV-1 subtype B infection between 2008 and 2013 were selected for next-generation sequencing of the HIV-1 envelope V3. Sequencing was performed on 2 plasma samples collected with an interval of > 6 months before the initiation of antiretroviral therapy. Maximum likelihood phylogenetic trees were inspected for dual infection, defined as the presence of two or more monophyletic clusters with ≥ 90% bootstrap support and a mean between-cluster genetic distance of ≥ 10%. To confirm dual infection, deep V3 sequencing of intermediate samples was performed as well as clonal sequencing of the HIV-1 protease-reverse transcriptase gene.

Results

Five of the 74 patients (6.8%) for whom deep sequencing was successful, showed clear evidence of dual infection. In 4 of them, the second strain was absent in the first sample but occurred in subsequent samples. This was highly suggestive for superinfection. In 3 patients both virus variants were of subtype B, in 2 patients at least one of the variants was a subtype B/non-B recombinant virus.

Conclusions

Dual infection was confirmed in 6.8% of MSM diagnosed with HIV-1 in Belgium. This prevalence is probably an underestimation, because stringent criteria were used to classify viral variants as originating from a new infection event.

SUBMITTER: Hebberecht L 

PROVIDER: S-EPMC5889168 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC7649213 | biostudies-literature
| S-EPMC3316524 | biostudies-other
| S-EPMC7219556 | biostudies-literature
| S-EPMC9749163 | biostudies-literature
| PRJEB69291 | ENA
| S-EPMC3499372 | biostudies-literature
| S-EPMC2702384 | biostudies-literature
| S-EPMC3338486 | biostudies-other
| S-EPMC8787144 | biostudies-literature
| S-EPMC3499670 | biostudies-other