Ontology highlight
ABSTRACT:
SUBMITTER: Ding K
PROVIDER: S-EPMC5906032 | biostudies-literature | 2018 Apr
REPOSITORIES: biostudies-literature
Ding Ke K Zhang Xing X Mrazek Jan J Kickhoefer Valerie A VA Lai Mason M Ng Hwee L HL Yang Otto O OO Rome Leonard H LH Zhou Z Hong ZH
Structure (London, England : 1993) 20180315 4
Prior crystal structures of the vault have provided clues of its structural variability but are non-conclusive due to crystal packing. Here, we obtained vaults by engineering at the N terminus of rat major vault protein (MVP) an HIV-1 Gag protein segment and determined their near-atomic resolution (∼4.8 Å) structures in a solution/non-crystalline environment. The barrel-shaped vaults in solution adopt two conformations, 1 and 2, both with D39 symmetry. From the N to C termini, each MVP monomer h ...[more]