Ontology highlight
ABSTRACT:
SUBMITTER: Niyonzima N
PROVIDER: S-EPMC5914421 | biostudies-literature | 2017 Jul
REPOSITORIES: biostudies-literature
Niyonzima Nixon N Lambert Abigail R AR Werther Rachel R De Silva Feelixge Harshana H Roychoudhury Pavitra P Greninger Alexander L AL Stone Daniel D Stoddard Barry L BL Jerome Keith R KR
Protein engineering, design & selection : PEDS 20170701 7
The combination of yeast surface display and flow cytometric analyses and selections is being used with increasing frequency to alter specificity of macromolecular recognition, including both protein-protein and protein-nucleic acid interactions. Here we describe the use of yeast surface display and cleavage-dependent flow cytometric assays to increase the specificity of an engineered meganuclease. The re-engineered meganuclease displays a significantly tightened specificity profile, while bindi ...[more]