Ontology highlight
ABSTRACT:
SUBMITTER: Lindenburg L
PROVIDER: S-EPMC8048591 | biostudies-literature | 2021 Apr
REPOSITORIES: biostudies-literature
Lindenburg Laurens L Hollfelder Florian F
Angewandte Chemie (International ed. in English) 20210308 16
NAD(H)-utiliing enzymes have been the subject of directed evolution campaigns to improve their function. To enable access to a larger swath of sequence space, we demonstrate the utility of a cell-free, ultrahigh-throughput directed evolution platform for dehydrogenases. Microbeads (1.5 million per sample) carrying both variant DNA and an immobilised analogue of NAD<sup>+</sup> were compartmentalised in water-in-oil emulsion droplets, together with cell-free expression mixture and enzyme substrat ...[more]