Ontology highlight
ABSTRACT:
SUBMITTER: Banala S
PROVIDER: S-EPMC5923430 | biostudies-literature | 2018 May
REPOSITORIES: biostudies-literature
Banala Sambashiva S Arvin Matthew C MC Bannon Nicholas M NM Jin Xiao-Tao XT Macklin John J JJ Wang Yong Y Peng Can C Zhao Guiqing G Marshall John J JJ Gee Kyle R KR Wokosin David L DL Kim Veronica J VJ McIntosh J Michael JM Contractor Anis A Lester Henry A HA Kozorovitskiy Yevgenia Y Drenan Ryan M RM Lavis Luke D LD
Nature methods 20180326 5
Photoactivatable pharmacological agents have revolutionized neuroscience, but the palette of available compounds is limited. We describe a general method for caging tertiary amines by using a stable quaternary ammonium linkage that elicits a red shift in the activation wavelength. We prepared a photoactivatable nicotine (PA-Nic), uncageable via one- or two-photon excitation, that is useful to study nicotinic acetylcholine receptors (nAChRs) in different experimental preparations and spatiotempor ...[more]