Dataset Information

Electrospun Composites of Polycaprolactone and Porous Silicon Nanoparticles for the Tunable Delivery of Small Therapeutic Molecules.

ABSTRACT: This report describes the use of an electrospun composite of poly(?-caprolactone) (PCL) fibers and porous silicon (pSi) nanoparticles (NPs) as an effective system for the tunable delivery of camptothecin (CPT), a small therapeutic molecule. Both materials are biodegradable, abundant, low-cost, and most importantly, have no known cytotoxic effects. The composites were treated with and without sodium hydroxide (NaOH) to investigate the wettability of the porous network for drug release and cell viability measurements. CPT release and subsequent cell viability was also investigated. We observed that the cell death rate was not only affected by the addition of our CPT carrier, pSi, but also by increasing the rate of dissolution via treatment with NaOH. This is the first example of loading pSi NPs as a therapeutics nanocarrier into electronspun PCL fibers and this system opens up new possibilities for the delivery of molecular therapeutics.

SUBMITTER: McInnes SJP

PROVIDER: S-EPMC5923535 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature

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Electrospun Composites of Polycaprolactone and Porous Silicon Nanoparticles for the Tunable Delivery of Small Therapeutic Molecules.

McInnes Steven J P SJP Macdonald Thomas J TJ Parkin Ivan P IP Nann Thomas T Voelcker Nicolas H NH

Nanomaterials (Basel, Switzerland) 20180329 4

This report describes the use of an electrospun composite of poly(ε-caprolactone) (PCL) fibers and porous silicon (pSi) nanoparticles (NPs) as an effective system for the tunable delivery of camptothecin (CPT), a small therapeutic molecule. Both materials are biodegradable, abundant, low-cost, and most importantly, have no known cytotoxic effects. The composites were treated with and without sodium hydroxide (NaOH) to investigate the wettability of the porous network for drug release and cell vi ...[more]

PMID: 29596352

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Project description:Biodegradable polycaprolactone/β-tricalcium phosphate (PT) composites are desirable candidates for bone tissue engineering applications. A higher β-tricalcium phosphate (TCP) ceramic content improves the mechanical, hydrophilic and osteogenic properties of PT scaffolds in vitro. Using a dynamic degradation reactor, we established a steady in vitro degradation model to investigate the changes in the physio-chemical and biological properties of PT scaffolds during degradation.PT46 and PT37 scaffolds underwent degradation more rapidly than PT scaffolds with lower TCP contents. In vivo studies revealed the rapid degradation of PT (PT46 and PT37) scaffolds disturbed macrophage responses and lead to bone healing failure. Macrophage co-culture assays and a subcutaneous implantation model indicated that the scaffold degradation process dynamically affected macrophage responses, especially polarization. RNA-Seq analysis indicated phagocytosis of the degradation products of PT37 scaffolds induces oxidative stress and inflammatory M1 polarization in macrophages. Overall, this study reveals that the dynamic patterns of biodegradation of degradable bone scaffolds highly orchestrate immune responses and thus determine the success of bone regeneration. Therefore, through evaluation of the biological effects of biomaterials during the entire process of degradation on immune responses and bone regeneration are necessary in order to develop more promising biomaterials for bone regeneration. Graphical abstract Schematic illustration of the mechanisms of scaffold degradation-mediated macrophage polarization and bone healing processes. A) Degradation of typical PT 55 scaffolds mediates sequential macrophage M1-M2 polarization and a matched “slow early-fast afterwards” bone regeneration mode. B) Degradation of typical PT37 scaffolds mediates ROS generation by macrophages, constant M1 polarization and a disordered “fast initially-slow in late stage” bone regeneration mode.Image 1 Highlights • This work found the vital role of bone scaffolds bio-degradation process in bone regenerative determination.• Excessive polycaprolactone/β-TCP scaffolds degradation disturbed sequential macrophages polarization.• Dynamic degradation model provided a way of investigation of degradation process of PCL/β-TCP scaffolds.• Phagocytosis of degradation products (DPs) by macrophages caused oxidative stress and M1 polarization in macrophages.

Dataset Information

Electrospun Composites of Polycaprolactone and Porous Silicon Nanoparticles for the Tunable Delivery of Small Therapeutic Molecules.

Publications

Electrospun Composites of Polycaprolactone and Porous Silicon Nanoparticles for the Tunable Delivery of Small Therapeutic Molecules.

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