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Quantitative proteomic profiling of primary cancer-associated fibroblasts in oesophageal adenocarcinoma.

ABSTRACT: BACKGROUND:Cancer-associated fibroblasts (CAFs) form the major stromal component of the tumour microenvironment (TME). The present study aimed to examine the proteomic profiles of CAFs vs. normal fibroblasts (NOFs) from patients with oesophageal adenocarcinoma to gain insight into their pro-oncogenic phenotype. METHODS:CAFs/NOFs from four patients were sub-cultured and analysed using quantitative proteomics. Differentially expressed proteins (DEPs) were subjected to bioinformatics and compared with published proteomics and transcriptomics  datasets. RESULTS:Principal component analysis of all profiled proteins showed that CAFs had high heterogeneity and clustered separately from NOFs. Bioinformatics interrogation of the DEPs demonstrated inhibition of adhesion of epithelial cells, adhesion of connective tissue cells and cell death of fibroblast cell lines in CAFs vs. NOFs (p?

SUBMITTER: Manousopoulou A 

PROVIDER: S-EPMC5943522 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature

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Quantitative proteomic profiling of primary cancer-associated fibroblasts in oesophageal adenocarcinoma.

Manousopoulou Antigoni A   Hayden Annette A   Mellone Massimiliano M   Garay-Baquero Diana J DJ   White Cory H CH   Noble Fergus F   Lopez Monette M   Thomas Gareth J GJ   Underwood Timothy J TJ   Garbis Spiros D SD  

British journal of cancer 20180329 9

<h4>Background</h4>Cancer-associated fibroblasts (CAFs) form the major stromal component of the tumour microenvironment (TME). The present study aimed to examine the proteomic profiles of CAFs vs. normal fibroblasts (NOFs) from patients with oesophageal adenocarcinoma to gain insight into their pro-oncogenic phenotype.<h4>Methods</h4>CAFs/NOFs from four patients were sub-cultured and analysed using quantitative proteomics. Differentially expressed proteins (DEPs) were subjected to bioinformatics  ...[more]

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