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Gene assembly via one-pot chemical ligation of DNA promoted by DNA nanostructures.

ABSTRACT: Current gene synthesis methods are driven by enzymatic reactions. Here we report the one-pot synthesis of a chemically-ligated gene from 14 oligonucleotides. The chemical ligation benefits from the highly efficient click chemistry approach templated by DNA nanostructures, and produces modified DNA that is compatible with polymerase enzymes.

SUBMITTER: Manuguerra I 

PROVIDER: S-EPMC5944424 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature

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Gene assembly via one-pot chemical ligation of DNA promoted by DNA nanostructures.

Manuguerra Ilenia I   Croce Stefano S   El-Sagheer Afaf H AH   Krissanaprasit Abhichart A   Brown Tom T   Gothelf Kurt V KV   Manetto Antonio A  

Chemical communications (Cambridge, England) 20180501 36

Current gene synthesis methods are driven by enzymatic reactions. Here we report the one-pot synthesis of a chemically-ligated gene from 14 oligonucleotides. The chemical ligation benefits from the highly efficient click chemistry approach templated by DNA nanostructures, and produces modified DNA that is compatible with polymerase enzymes. ...[more]

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