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Long-wavelength TCF-based fluorescence probes for the detection and intracellular imaging of biological thiols.

ABSTRACT: Two 'turn on' TCF-based fluorescence probes were developed for the detection of biological thiols (TCF-GSH and TCFCl-GSH). TCF-GSH was shown to have a high sensitivity towards glutathione (GSH) with a 0.28 ?M limit of detection. Unfortunately, at higher GSH concentrations the fluorescence intensity of TCF-GSH decreased and toxicity was observed for TCF-GSH in live cells. However, TCFCl-GSH was shown to be able to detect GSH at biologically relevant concentrations with a 0.45 ?M limit of detection. No toxicity was found for TCFCl-GSH and a clear 'turn on' with good photostability was observed for the exogenous addition of GSH, Cys and HCys. Furthermore, TCFCl-GSH was used to evaluate the effects of drug treatment on the levels of GSH in live cells.

SUBMITTER: Sedgwick AC

PROVIDER: S-EPMC5944426 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature

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Long-wavelength TCF-based fluorescence probes for the detection and intracellular imaging of biological thiols.

Sedgwick Adam C AC Gardiner Jordan E JE Kim Gyoungmi G Yevglevskis Maksims M Lloyd Matthew D MD Jenkins A Toby A ATA Bull Steven D SD Yoon Juyoung J James Tony D TD

Chemical communications (Cambridge, England) 20180501 38

Two 'turn on' TCF-based fluorescence probes were developed for the detection of biological thiols (TCF-GSH and TCFCl-GSH). TCF-GSH was shown to have a high sensitivity towards glutathione (GSH) with a 0.28 μM limit of detection. Unfortunately, at higher GSH concentrations the fluorescence intensity of TCF-GSH decreased and toxicity was observed for TCF-GSH in live cells. However, TCFCl-GSH was shown to be able to detect GSH at biologically relevant concentrations with a 0.45 μM limit of detectio ...[more]

PMID: 29683468

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