Dataset Information

Human T cell responses to Dengue and Zika virus infection compared to Dengue/Zika coinfection.

ABSTRACT:

Introduction

Zika virus (ZIKV) and dengue virus (DENV) co-circulated during latest outbreaks in Brazil, hence, it is important to evaluate the host cross-reactive immune responses to these viruses. So far, little is known about human T cell responses to ZIKV and no reports detail adaptive immune responses during DENV/ZIKV coinfection.

Methods

Here, we studied T cells responses in well-characterized groups of DENV, ZIKV, or DENV/ZIKV infected patients and DENV-exposed healthy donors. We evaluated chemokine receptors expression and single/multifunctional frequencies of IFN?, TNF, and IL2-producing T cells during these infections. Even without antigenic stimulation, it was possible to detect chemokine receptors and IFN?, TNF, and IL2-producing T cells from all individuals by flow cytometry. Additionally, PBMCs' IFN? response to DENV NS1 protein and to polyclonal stimuli was evaluated by ELISPOT.

Results

DENV and ZIKV infections and DENV/ZIKV coinfections similarly induced expression of CCR5, CX3CR1, and CXCR3 on CD4 and CD8 T cells. DENV/ZIKV coinfection decreased the ability of CD4⁺ T cells to produce IFN?⁺ , TNF⁺ , TNF?^+? IFN?⁺ , and TNF?⁺ ?IL2⁺ , compared to DENV and ZIKV infections. A higher magnitude of IFN? response to DENV NS1 was found in donors with a history of dengue infection, however, a hyporesponsiveness was found in acute DENV, ZIKV, or DENV/ZIKV infected patients, even previously infected with DENV.

Conclusion

Therefore, we emphasize the potential impact of coinfection on the immune response from human hosts, mainly in areas where DENV and ZIKV cocirculate.

SUBMITTER: Badolato-Correa J

PROVIDER: S-EPMC5946158 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature

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Publications

Human T cell responses to Dengue and Zika virus infection compared to Dengue/Zika coinfection.

Badolato-Corrêa Jessica J Sánchez-Arcila Juan Camilo JC Alves de Souza Thiara Manuele TM Santos Barbosa Luciana L Conrado Guerra Nunes Priscila P da Rocha Queiroz Lima Monique M Gandini Mariana M Bispo de Filippis Ana Maria AM Venâncio da Cunha Rivaldo R Leal de Azeredo Elzinandes E de-Oliveira-Pinto Luzia Maria LM

Immunity, inflammation and disease 20171228 2

<h4>Introduction</h4>Zika virus (ZIKV) and dengue virus (DENV) co-circulated during latest outbreaks in Brazil, hence, it is important to evaluate the host cross-reactive immune responses to these viruses. So far, little is known about human T cell responses to ZIKV and no reports detail adaptive immune responses during DENV/ZIKV coinfection.<h4>Methods</h4>Here, we studied T cells responses in well-characterized groups of DENV, ZIKV, or DENV/ZIKV infected patients and DENV-exposed healthy donor ...[more]

PMID: 29282904

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Project description:BackgroundMosquito-borne arboviruses are expanding their territory and elevating their infection prevalence due to the rapid climate change, urbanization, and increased international travel and global trade. Various significant arboviruses, including the dengue virus, Zika virus, Chikungunya virus, and yellow fever virus, are all reliant on the same primary vector, Aedes aegypti. Consequently, the occurrence of arbovirus coinfection in mosquitoes is anticipated. Arbovirus coinfection in mosquitoes has two patterns: simultaneous and sequential. Numerous studies have demonstrated that simultaneous coinfection of arboviruses in mosquitoes is unlikely to exert mutual developmental influence on these viruses. However, the viruses' interplay within a mosquito after the sequential coinfection seems intricated and not well understood.Methodology/principal findingsWe conducted experiments aimed at examining the phenomenon of arbovirus sequential coinfection in both mosquito cell line (C6/36) and A. aegypti, specifically focusing on dengue virus (DENV, serotype 2) and Zika virus (ZIKV). We firstly observed that DENV and ZIKV can sequentially infect mosquito C6/36 cell line, but the replication level of the subsequently infected ZIKV was significantly suppressed. Similarly, A. aegypti mosquitoes can be sequentially coinfected by these two arboviruses, regardless of the order of virus exposure. However, the replication, dissemination, and the transmission potential of the secondary virus were significantly inhibited. We preliminarily explored the underlying mechanisms, revealing that arbovirus-infected mosquitoes exhibited activated innate immunity, disrupted lipid metabolism, and enhanced RNAi pathway, leading to reduced susceptibility to the secondary arbovirus infections.Conclusions/significanceOur findings suggest that, in contrast to simultaneous arbovirus coinfection in mosquitoes that can promote the transmission and co-circulation of these viruses, sequential coinfection appears to have limited influence on arbovirus transmission dynamics. However, it is important to note that more experimental investigations are needed to refine and expand upon this conclusion.

Dataset Information

Human T cell responses to Dengue and Zika virus infection compared to Dengue/Zika coinfection.

Introduction

Methods

Results

Conclusion

Publications

Human T cell responses to Dengue and Zika virus infection compared to Dengue/Zika coinfection.

Similar Datasets

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