Ontology highlight
ABSTRACT:
SUBMITTER: Suter-Crazzolara C
PROVIDER: S-EPMC5952203 | biostudies-literature | 1991 May
REPOSITORIES: biostudies-literature
Suter-Crazzolara C C Keller W W
Gene expression 19910501 2
The nucleotide sequence of U11 small nuclear RNA, a minor U RNA from HeLa cells, was determined. Computer analysis of the sequence (135 residues) predicts two strong hairpin loops which are separated by seventeen nucleotides containing an Sm binding site (AAUUUUUUGG). A synthetic gene was constructed in which the coding region of U11 RNA is under the control of a T7 promoter. This vector can be used to produce U11 RNA in vitro. Southern hybridization and PCR analysis of HeLa genomic DNA suggest ...[more]