Ontology highlight
ABSTRACT:
SUBMITTER: Seebald LM
PROVIDER: S-EPMC5956527 | biostudies-literature | 2017 May
REPOSITORIES: biostudies-literature
Seebald Leah M LM DeMott Christopher M CM Ranganathan Srivathsan S Asare-Okai Papa Nii PN Glazunova Anastasia A Chen Alan A Shekhtman Alexander A Royzen Maksim M
Journal of inorganic biochemistry 20170224
Paramagnetic resonance enhancement (PRE) is an NMR technique that allows studying three-dimensional structures of RNA-protein complexes in solution. RNA strands are typically spin labeled using nitroxide reagents, which provide minimal perturbation to the native structure. The current work describes an alternative approach, which is based on a Co<sup>2+</sup>-based probe that can be covalently attached to RNA in the vicinity of the protein's binding site using 'click' chemistry. Similar to nitro ...[more]