Ontology highlight
ABSTRACT:
SUBMITTER: Seebald LM
PROVIDER: S-EPMC5956532 | biostudies-literature | 2017 Apr
REPOSITORIES: biostudies-literature
Seebald Leah M LM DeMott Christopher M CM Ranganathan Srivathsan S Asare Okai Papa Nii PN Glazunova Anastasia A Chen Alan A Shekhtman Alexander A Royzen Maksim M
Inorganic chemistry 20170322 7
Paramagnetic NMR techniques allow for studying three-dimensional structures of RNA-protein complexes. In particular, paramagnetic relaxation enhancement (PRE) data can provide valuable information about long-range distances between different structural components. For PRE NMR experiments, oligonucleotides are typically spin-labeled using nitroxide reagents. The current work describes an alternative approach involving a Cu(II) cyclen-based probe that can be covalently attached to an RNA strand in ...[more]