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ABSTRACT:
SUBMITTER: Saio T
PROVIDER: S-EPMC3193990 | biostudies-literature | 2011 Nov
REPOSITORIES: biostudies-literature
Saio Tomohide T Ogura Kenji K Shimizu Kazumi K Yokochi Masashi M Burke Terrence R TR Inagaki Fuyuhiko F
Journal of biomolecular NMR 20110917 3
A nuclear magnetic resonance-based ligand screening strategy utilizing a paramagnetic lanthanide probe is presented. By fixing a paramagnetic lanthanide ion to a target protein, a pseudo-contact shift (PCS) and a paramagnetic relaxation enhancement (PRE) can be observed for both the target protein and its bound ligand. Based on PRE and PCS information, the bound ligand is then screened from the compound library and the structure of the ligand-protein complex is determined. PRE is an isotropic pa ...[more]