Ontology highlight
ABSTRACT:
SUBMITTER: Sosio M
PROVIDER: S-EPMC5959762 | biostudies-literature | 2018 May
REPOSITORIES: biostudies-literature
Sosio Margherita M Gaspari Eleonora E Iorio Marianna M Pessina Silvia S Medema Marnix H MH Bernasconi Alice A Simone Matteo M Maffioli Sonia I SI Ebright Richard H RH Donadio Stefano S
Cell chemical biology 20180315 5
Pseudouridimycin (PUM) is a selective nucleoside-analog inhibitor of bacterial RNA polymerase with activity against Gram-positive and Gram-negative bacteria. PUM, produced by Streptomyces sp. ID38640, consists of a formamidinylated, N-hydroxylated Gly-Gln dipeptide conjugated to 5'-aminopseudouridine. We report the characterization of the PUM gene cluster. Bioinformatic analysis and mutational knockouts of pum genes with analysis of accumulated intermediates, define the PUM biosynthetic pathway. ...[more]