Ontology highlight
ABSTRACT:
SUBMITTER: Hiener B
PROVIDER: S-EPMC5960642 | biostudies-literature | 2017 Oct
REPOSITORIES: biostudies-literature
Hiener Bonnie B Horsburgh Bethany A BA Eden John-Sebastian JS Barton Kirston K Schlub Timothy E TE Lee Eunok E von Stockenstrom Susanne S Odevall Lina L Milush Jeffrey M JM Liegler Teri T Sinclair Elizabeth E Hoh Rebecca R Boritz Eli A EA Douek Daniel D Fromentin Rémi R Chomont Nicolas N Deeks Steven G SG Hecht Frederick M FM Palmer Sarah S
Cell reports 20171001 3
Latent replication-competent HIV-1 persists in individuals on long-term antiretroviral therapy (ART). We developed the Full-Length Individual Proviral Sequencing (FLIPS) assay to determine the distribution of latent replication-competent HIV-1 within memory CD4<sup>+</sup> T cell subsets in six individuals on long-term ART. FLIPS is an efficient, high-throughput assay that amplifies and sequences near full-length (∼9 kb) HIV-1 proviral genomes and determines potential replication competency thro ...[more]