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P2Y12R-Dependent Translocation Mechanisms Gate the Changing Microglial Landscape.

ABSTRACT: Microglia are an exquisitely tiled and self-contained population in the CNS that do not receive contributions from circulating monocytes in the periphery. While microglia are long-lived cells, the extent to which their cell bodies are fixed and the molecular mechanisms by which the microglial landscape is regulated have not been determined. Using chronic in vivo two-photon imaging to follow the microglial population in young adult mice, we document a daily rearrangement of the microglial landscape. Furthermore, we show that the microglial landscape can be modulated by severe seizures, acute injury, and sensory deprivation. Finally, we demonstrate a critical role for microglial P2Y12Rs in regulating the microglial landscape through cellular translocation independent of proliferation. These findings suggest that microglial patrol the CNS through both process motility and soma translocation.

SUBMITTER: Eyo UB

PROVIDER: S-EPMC5965271 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature

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P2Y12R-Dependent Translocation Mechanisms Gate the Changing Microglial Landscape.

Eyo Ukpong B UB Mo Mingshu M Yi Min-Hee MH Murugan Madhuvika M Liu Junting J Yarlagadda Rohan R Margolis David J DJ Xu Pingyi P Wu Long-Jun LJ

Cell reports 20180401 4

Microglia are an exquisitely tiled and self-contained population in the CNS that do not receive contributions from circulating monocytes in the periphery. While microglia are long-lived cells, the extent to which their cell bodies are fixed and the molecular mechanisms by which the microglial landscape is regulated have not been determined. Using chronic in vivo two-photon imaging to follow the microglial population in young adult mice, we document a daily rearrangement of the microglial landsca ...[more]

PMID: 29694903

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