Ontology highlight
ABSTRACT:
SUBMITTER: Gamarra N
PROVIDER: S-EPMC5976439 | biostudies-literature | 2018 Apr
REPOSITORIES: biostudies-literature
Gamarra Nathan N Johnson Stephanie L SL Trnka Michael J MJ Burlingame Alma L AL Narlikar Geeta J GJ
eLife 20180417
ISWI family chromatin remodeling motors use sophisticated autoinhibition mechanisms to control nucleosome sliding. Yet how the different autoinhibitory domains are regulated is not well understood. Here we show that an acidic patch formed by histones H2A and H2B of the nucleosome relieves the autoinhibition imposed by the AutoN and the NegC regions of the human ISWI remodeler SNF2h. Further, by single molecule FRET we show that the acidic patch helps control the distance travelled per translocat ...[more]