Super-resolution force spectroscopy reveals ribosomal motion at sub-nucleotide steps.
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ABSTRACT:
SUBMITTER: Jia H
PROVIDER: S-EPMC5989005 | biostudies-literature | 2018 Jun
REPOSITORIES: biostudies-literature
Publications
Super-resolution force spectroscopy reveals ribosomal motion at sub-nucleotide steps.
Jia Haina H Wang Yuhong Y Xu Shoujun S
Chemical communications (Cambridge, England) 20180601 46
Probing biomolecular motion beyond a single nucleotide is technically challenging but fundamentally significant. We have developed super-resolution force spectroscopy (SURFS) with 0.5 pN force resolution and revealed that the ribosome moves by half a nucleotide upon the formation of the pre-translocation complex, which is beyond the resolution of other techniques. ...[more]
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