Dataset Information

Super-Resolution Imaging Reveals Dynamic Reticular Cytoophidia.

ABSTRACT: CTP synthase (CTPS) can form filamentous structures termed cytoophidia in cells in all three domains of life. In order to study the mesoscale structure of cytoophidia, we perform fluorescence recovery after photobleaching (FRAP) and stimulated emission depletion (STED) microscopy in human cells. By using an EGFP dimeric tag as a tool to explore the physical properties of cytoophidia, we find that cytoophidia are dynamic and reticular. The reticular structure of CTPS cytoophidia may provide space for other components, such as IMPDH. In addition, we observe CTPS granules with tentacles.

SUBMITTER: Fang YF

PROVIDER: S-EPMC9569780 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature

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Super-Resolution Imaging Reveals Dynamic Reticular Cytoophidia.

Fang Yi-Fan YF Li Yi-Lan YL Li Xiao-Ming XM Liu Ji-Long JL

International journal of molecular sciences 20221002 19

CTP synthase (CTPS) can form filamentous structures termed cytoophidia in cells in all three domains of life. In order to study the mesoscale structure of cytoophidia, we perform fluorescence recovery after photobleaching (FRAP) and stimulated emission depletion (STED) microscopy in human cells. By using an EGFP dimeric tag as a tool to explore the physical properties of cytoophidia, we find that cytoophidia are dynamic and reticular. The reticular structure of CTPS cytoophidia may provide space ...[more]

PMID: 36233000

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Dataset Information

Super-Resolution Imaging Reveals Dynamic Reticular Cytoophidia.

Publications

Super-Resolution Imaging Reveals Dynamic Reticular Cytoophidia.

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