Ontology highlight
ABSTRACT:
SUBMITTER: Colicino EG
PROVIDER: S-EPMC6004580 | biostudies-literature | 2018 Mar
REPOSITORIES: biostudies-literature
Colicino Erica G EG Garrastegui Alice M AM Freshour Judy J Santra Peu P Post Dawn E DE Kotula Leszek L Hehnly Heidi H
Molecular biology of the cell 20171227 5
We propose to understand how the mitotic kinase PLK1 drives chromosome segregation errors, with a specific focus on Gravin, a PLK1 scaffold. In both three-dimensional primary prostate cancer cell cultures that are prone to Gravin depletion and Gravin short hairpin RNA (shRNA)-treated cells, an increase in cells containing micronuclei was noted in comparison with controls. To examine whether the loss of Gravin affected PLK1 distribution and activity, we utilized photokinetics and a PLK1 activity ...[more]