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Labeling and identifying cell-specific proteomes in the mouse brain.

ABSTRACT: We develop an approach to tag proteomes synthesized by specific cell types in dissociated cortex, brain slices, and the brains of live mice. By viral-mediated expression of an orthogonal pyrrolysyl-tRNA synthetase-tRNAXXX pair in a cell type of interest and providing a non-canonical amino acid with a chemical handle, we selectively label neuronal or glial proteomes. The method enables the identification of proteins from spatially and genetically defined regions of the brain.

SUBMITTER: Krogager TP

PROVIDER: S-EPMC6010150 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature

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Labeling and identifying cell-specific proteomes in the mouse brain.

Krogager Toke P TP Ernst Russell J RJ Elliott Thomas S TS Calo Laura L Beránek Václav V Ciabatti Ernesto E Spillantini Maria Grazia MG Tripodi Marco M Hastings Michael H MH Chin Jason W JW

Nature biotechnology 20171218 2

We develop an approach to tag proteomes synthesized by specific cell types in dissociated cortex, brain slices, and the brains of live mice. By viral-mediated expression of an orthogonal pyrrolysyl-tRNA synthetase-tRNAXXX pair in a cell type of interest and providing a non-canonical amino acid with a chemical handle, we selectively label neuronal or glial proteomes. The method enables the identification of proteins from spatially and genetically defined regions of the brain. ...[more]

PMID: 29251727

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