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Labelling and identifying cell-specific proteomes in the mouse brain

ABSTRACT: Defining the in vivo proteomes of specific cell-types in the central nervous system of mammals, without ex vivo manipulation, is an important and outstanding challange. Here we demonstrate that stochastic orthogonal recoding of translation (SORT) can be used to tag the proteomes of specific cell types in brains of live mice. We tag neuronal proteomes from the striatum and perform enrichment before identifing neuronal proteins by LC-MSMS.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Striatal Neuron

SUBMITTER: Toke Hansen

LAB HEAD: Jason Chin

PROVIDER: PXD008380 | Pride | 2017-12-13

REPOSITORIES: pride

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			Action	DRS
	ControlSORT_1A.raw	Raw
	ControlSORT_1B.raw	Raw
	ControlSORT_2A.raw	Raw
	ControlSORT_2B.raw	Raw
	ControlSORT_3A.raw	Raw

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Labeling and identifying cell-specific proteomes in the mouse brain.

Krogager Toke P TP Ernst Russell J RJ Elliott Thomas S TS Calo Laura L Beránek Václav V Ciabatti Ernesto E Spillantini Maria Grazia MG Tripodi Marco M Hastings Michael H MH Chin Jason W JW

Nature biotechnology 20171218 2

We develop an approach to tag proteomes synthesized by specific cell types in dissociated cortex, brain slices, and the brains of live mice. By viral-mediated expression of an orthogonal pyrrolysyl-tRNA synthetase-tRNAXXX pair in a cell type of interest and providing a non-canonical amino acid with a chemical handle, we selectively label neuronal or glial proteomes. The method enables the identification of proteins from spatially and genetically defined regions of the brain. ...[more]

PMID: 29251727

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